FastQCFastQC Report
Mon 6 Feb 2017
SRR2079254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209173
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65300.038.3556141
TCAACGC84200.029.2134534
TATCAAC86600.028.8818872
ATCAACG85600.028.7356643
CAACGCA86300.028.4626065
AACGCAG89900.027.437966
GGTATCA28200.024.711991
ACGCAGA104750.023.1859077
AGAGTAC98550.022.75352311
CGCAGAG106450.022.7501038
CAGAGTA112200.020.44658310
GAGTACT43450.020.32585712
GCAGAGT115950.020.1720899
GTACATG114750.019.3614851
TACATGG115950.018.8040682
AGTACTT48350.018.48000113
GTACTTT47750.018.27929314
ACATGGG119500.017.6103273
GTATATA13900.016.8771691
GTATAAT13850.015.6926481
Produced by FastQC (version 0.11.5)