FastQCFastQC Report
Mon 6 Feb 2017
SRR2079254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209173
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81550.040.6374321
GGTATCA35350.030.2065161
TATCAAC111700.029.575792
ATCAACG112050.029.298363
TCAACGC112850.029.1779774
CAACGCA115450.028.51825
AACGCAG118900.027.8004346
ACGCAGA135750.024.1184487
CGCAGAG138750.023.5178348
AGAGTAC132450.023.33323311
GAGTACT80450.022.59453412
CGTACTA15100.021.70028929
CAGAGTA145450.021.69832410
ACCGTAC15800.021.612127
AGTACTT85700.021.57261713
GCAGAGT149250.021.2845349
GTACTTT87150.020.6991814
TCTCGTA15750.020.1477338
GACCGTA16850.020.06065226
GTACATG111400.020.0081141
Produced by FastQC (version 0.11.5)