FastQCFastQC Report
Mon 6 Feb 2017
SRR2079253_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079253_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764107
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12500.039.4677351
GGTATCA5100.034.499771
TATCAAC17000.028.2086372
TCAACGC17850.027.4451964
ATCAACG17800.027.3284683
CAACGCA18250.026.6546175
TAACGGG658.6547656E-426.5382863
AACGCAG19250.025.4491836
GATAACG902.125544E-422.9998461
GTATAGT1554.082176E-822.2579151
ACGCAGA22450.021.6680077
CGCAGAG22800.021.4866988
GAGTACT13700.020.39767512
AGAGTAC23350.019.65083311
GTACATG21450.019.1397341
TACATGG20850.018.8631842
GTAGGGG1852.640336E-718.6485253
AGTACTT15750.018.3998813
CCGTACA950.00783240918.1577762
CATGGGG12800.018.0584744
Produced by FastQC (version 0.11.5)