FastQCFastQC Report
Mon 6 Feb 2017
SRR2079253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764107
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.18910964040376543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.10090209878982918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAT306.3185015E-445.987619
GTATCAA20400.043.2095151
GGTATCA7550.039.8328821
TATCAAC27350.031.8502332
CGCTATA553.2611607E-431.35518833
TCAACGC27600.031.3030344
ATCAACG28200.030.767633
CAACGCA28050.030.6756325
TATCTCG7500.029.89194936
GCCGTCT7750.029.81777646
TGCCGTC7600.029.49863245
AACGCAG29300.029.3611686
ACGCTAT8250.029.26484532
TATGCCG7850.028.99855843
TCGTATG8150.028.77752340
ATGCCGT7850.028.55918744
CGCTATC7750.028.4826533
CCGTCTT8200.028.18143347
TCTCGTA7750.028.0376138
CTCGTAT7700.027.7717439
Produced by FastQC (version 0.11.5)