FastQCFastQC Report
Mon 6 Feb 2017
SRR2079252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439898
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77800.035.5181121
TCAACGC99450.027.0574444
ATCAACG101700.026.492753
TATCAAC103950.026.2843762
CAACGCA103350.026.1365495
AACGCAG106650.025.2631286
GGTATCA38800.021.9615121
ACGCAGA123400.021.5264557
CGCAGAG127500.020.698488
AGAGTAC117050.020.60214811
GAGTACT52850.020.04033712
GTACATG132550.019.3637811
CAGAGTA135950.018.65091710
TACATGG135000.018.4501722
GCAGAGT141550.018.1080239
AGTACTT59700.017.97185113
GTACTTT58950.017.61646514
CGAATCG3153.6379788E-1217.52469337
ACATGGG142050.016.8059023
CGATCGT3552.910383E-1115.54955542
Produced by FastQC (version 0.11.5)