FastQCFastQC Report
Mon 6 Feb 2017
SRR2079252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439898
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101150.039.344981
GGTATCA45050.030.29041
TATCAAC136550.028.9172632
TCAACGC139300.028.168984
ATCAACG141450.027.8178443
CAACGCA141650.027.774185
AACGCAG148750.026.5389776
ACGCAGA168100.023.277047
GAGTACT107300.022.43793712
CGCAGAG174250.022.251288
AGAGTAC167250.021.92271611
AGTACTT114650.021.11982313
GTACTTT114800.020.76172314
CAGAGTA187100.019.85496510
GCAGAGT193200.019.4958749
TCTCGTA15050.019.25174138
CTCGTAT15250.019.22544139
GTACATG130900.019.0546171
TATGCCG15700.018.8940943
TACTTTT126550.018.764315
Produced by FastQC (version 0.11.5)