FastQCFastQC Report
Mon 6 Feb 2017
SRR2079251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942537
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72100.040.04941
GGTATCA30300.032.3356481
TCAACGC98150.028.6817534
ATCAACG98900.028.5340163
TATCAAC100150.028.350112
CAACGCA99600.028.1949215
AACGCAG104550.026.8600126
ACGCAGA122450.022.7081687
CGCAGAG127850.021.6411088
AGAGTAC126900.020.79744711
GTACATG147750.020.0339071
TACATGG146600.019.2497542
CAGAGTA139250.019.17571610
GAGTACT60650.018.82852212
GCAGAGT145800.018.4562289
ACATGGG153950.017.9273513
AGTACTT68900.016.8243813
GTACTTT69000.016.30049114
CATGGGG112350.015.967474
GTATAAT17500.015.7709471
Produced by FastQC (version 0.11.5)