FastQCFastQC Report
Mon 6 Feb 2017
SRR2079251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942537
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102400.041.6966061
GGTATCA39650.039.205911
ATCAACG143750.029.6063613
TCAACGC144450.029.3612824
TATCAAC145100.029.3309062
CAACGCA148550.028.480585
AACGCAG153850.027.5185536
ACGCAGA178200.023.485817
CGCAGAG181950.022.9969888
TATCTCG10800.022.99536136
AGAGTAC183250.021.83461211
GAGTACT124650.021.22435812
CAGAGTA195550.020.67289410
GCAGAGT202050.020.3151289
CTCGTAT11950.020.20512839
AGTACTT131050.020.18783813
ACGGACT13250.019.52436327
GTACATG140900.019.4666731
GTACTTT135850.019.39836714
TACATGG142000.018.5617282
Produced by FastQC (version 0.11.5)