FastQCFastQC Report
Mon 6 Feb 2017
SRR2079250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295317
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63400.039.8306471
GGTATCA27750.031.0793951
TCAACGC81700.030.1066884
ATCAACG82500.029.8147433
TATCAAC85150.029.3325252
CAACGCA84450.029.2488545
AACGCAG88450.028.0431356
ACGCAGA102250.024.0896457
CGCAGAG106400.022.890148
AGAGTAC107300.021.28402511
GTACATG124450.020.3191051
CAGAGTA114700.020.24123610
GCAGAGT118350.019.8210229
TACATGG125100.019.6622
GAGTACT53100.019.23104112
ACATGGG129550.018.3475173
AGTACTT61750.016.92821913
CATGGGG86150.015.7373924
GTACTTT63200.015.612946514
GTGTAGC21550.014.7277381
Produced by FastQC (version 0.11.5)