FastQCFastQC Report
Mon 6 Feb 2017
SRR2079250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295317
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93000.042.6573331
GGTATCA33600.038.362271
TCAACGC128150.030.7638724
ATCAACG129000.030.6190173
TATCAAC129200.030.4646342
CAACGCA132500.029.7243445
AACGCAG136500.028.9012286
ACGCAGA160550.024.4623157
CGCAGAG164850.023.7290048
GAGTACT113350.023.18693512
TTACGCG902.1312971E-422.99421943
AGAGTAC165900.022.72394411
CAGAGTA173150.022.01150310
AGTACTT121050.021.76913
GCAGAGT180400.021.260739
GTACTTT126800.020.61863314
GTACATG125150.019.6600151
TACATGG124450.019.1874772
TACTTTT144850.018.25319115
ACTTTTT149300.017.9634516
Produced by FastQC (version 0.11.5)