FastQCFastQC Report
Mon 6 Feb 2017
SRR2079249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775881
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48350.042.025311
TCAACGC60550.032.304354
ATCAACG62250.031.6438163
CAACGCA62550.031.2714395
TATCAAC65600.030.5011542
AACGCAG66200.029.5472586
GGTATCA18750.027.966261
ACGCAGA76400.025.6024677
CGCAGAG77700.024.7301248
AGAGTAC78500.022.72024311
CAGAGTA82550.021.77272410
GAGTACT39900.021.7020512
GTACATG90050.021.6449621
TACATGG89400.021.1849252
GCAGAGT87000.020.9762849
AGTACTT42600.020.4075513
GTACTTT42150.020.13470514
ACATGGG96500.019.1257553
GTATTAC5350.018.0549531
CATGGGG62600.017.5795784
Produced by FastQC (version 0.11.5)