FastQCFastQC Report
Mon 6 Feb 2017
SRR2079249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775881
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68550.043.5017431
GGTATCA25250.038.044051
ATCAACG92350.031.7668483
TATCAAC93550.031.5809822
TCAACGC92800.031.5315064
CAACGCA95300.030.667545
AACGCAG99550.029.3871466
CGCGCGA500.007745363327.59372536
ACGCAGA112550.025.683617
CGCAGAG117750.024.5197568
AGAGTAC117600.023.37605911
GAGTACT72500.022.93133712
GCAGAGT126800.022.1968449
CAGAGTA126500.022.08588610
AGTACTT76750.022.0659913
GTACTTT78350.021.21916814
CTCGTAT10700.020.30846639
CGTACTA850.004127057720.28950312
AATCTCG10800.019.80105236
TATGCCG11300.019.53538143
Produced by FastQC (version 0.11.5)