FastQCFastQC Report
Mon 6 Feb 2017
SRR2079248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605094
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41800.040.439471
GGTATCA16700.031.1922051
TCAACGC55100.029.8016954
ATCAACG55300.029.6939133
TATCAAC56600.029.560442
CAACGCA56850.028.8236375
AACGCAG58950.027.7968436
ACGCAGA68700.023.6008017
CGCAGAG68900.023.2318828
AGAGTAC68300.021.97122211
CAGAGTA74900.020.21940210
GCAGAGT77050.020.1924749
GTACATG75750.019.9014361
TACATGG76400.019.009662
GAGTACT33050.018.89336812
ACATGGG78100.017.6241233
AGTACTT36250.017.3207113
GTATAGG7650.017.1359351
GTACTTT36150.016.98852514
GTATAGC7950.016.0553671
Produced by FastQC (version 0.11.5)