FastQCFastQC Report
Mon 6 Feb 2017
SRR2079248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605094
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55550.043.3452761
GGTATCA19600.038.7757031
TATCAAC76800.031.1720282
TCAACGC77100.031.046564
ATCAACG77000.031.0013333
CAACGCA78400.030.3995865
AACGCAG81400.029.5315386
CGAATCG854.438398E-628.40565737
ACGCAGA92950.025.4150687
CGCAGAG95800.024.558098
AGAGTAC97550.022.98326711
GAGTACT66400.022.75263612
CAGAGTA102150.022.15088810
AGTACTT69850.021.7769913
TACCGCG800.00289272721.57235517
GCAGAGT105600.021.5578639
CGTACTA12950.021.30815929
GTACTTT70750.021.20745314
ACCGTAC13600.020.28975527
TATGCCG13450.020.25958343
Produced by FastQC (version 0.11.5)