FastQCFastQC Report
Mon 6 Feb 2017
SRR2079247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465805
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT450.00462320730.66769419
GTATCAA93350.029.3806481
ATCAACG110700.024.0278723
TCAACGC112050.023.7999574
TATCAAC114300.023.663472
GAGTACT62300.023.64549312
CAACGCA113150.023.5685845
AACGCAG115350.023.1788926
TATACCG902.1279056E-422.9994435
CGCGTAA902.1281136E-422.9991165
AGAGTAC106500.022.44875111
AGTACTT66850.022.03611613
GTACTTT68300.021.51871114
ACGCAGA127050.020.8271227
CGCAGAG127500.020.6453828
GTATATA19000.019.97321
CGCAATC2102.3555913E-919.71352463
GTACATG93200.019.4705581
TACATGG91650.018.7835042
GCAGAGT137600.018.4279699
Produced by FastQC (version 0.11.5)