FastQCFastQC Report
Mon 6 Feb 2017
SRR2079247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465805
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.10176567925777706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129350.032.7193761
GGCGAAT9950.025.99949632
GCGAATC9350.025.82338533
TATCAAC164050.025.7353632
ATCAACG163900.025.6956773
TCAACGC166300.025.2993144
CAACGCA169750.024.8243965
AACGCAG173450.024.2525676
ACGTAAT4000.024.14486556
GAGTACT126350.023.83228712
AGAGTAC167550.023.42742511
TATGCCG10900.023.41703843
GGTATCA68050.023.404971
CTCGTAT10500.023.3236139
TCGTATG10950.023.31011240
AATTCGT3700.023.30585560
AGTACTT130850.023.03904313
AGGCGAA11800.022.21561431
ACGCAGA188500.022.1683647
CGAATCT10750.022.13947734
Produced by FastQC (version 0.11.5)