FastQCFastQC Report
Mon 6 Feb 2017
SRR2079244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843869
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35950.033.491461
GGTATCA18350.031.298661
TCAACGC45100.026.388754
ATCAACG46300.026.1518483
TATCAAC46200.026.0068632
CAACGCA46900.024.934645
AACGCAG49900.022.9516456
TATACCG1551.0064887E-620.0302815
ACGCAGA56950.019.321587
GTACATG57750.019.2911261
CGAATCG1108.2540716E-418.81367737
CGCAGAG57950.018.4524468
AGAGTAC53200.017.83659411
TACATGG59800.017.0899662
GAGTACT28700.015.749433512
ACATGGG64200.015.7437743
CAGAGTA60350.015.723392510
GCAGAGT64150.015.1682559
TATACTG10700.014.8303135
TCCGCGA1400.004113350514.79622645
Produced by FastQC (version 0.11.5)