FastQCFastQC Report
Mon 6 Feb 2017
SRR2079244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843869
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61600.041.886911
GGTATCA24500.039.105141
TATCAAC88350.028.9487172
ATCAACG91350.028.4136473
TCAACGC91850.027.9959244
CAACGCA95200.026.865755
AACGCAG97700.026.2186976
ACGCAGA113800.022.0772867
CGCAGAG114400.021.7453568
AGAGTAC111900.020.50412211
GAGTACT83700.020.1161112
CAGAGTA118450.019.69070410
AGTACTT87850.019.31885313
GCAGAGT123150.019.1633459
GTACATG60500.018.8659521
GTACTTT89800.018.788414
TACATGG62600.017.8092612
ACATGGG65550.016.5338753
TACTTTT103350.016.35847715
ACTTTTT109150.016.02660416
Produced by FastQC (version 0.11.5)