FastQCFastQC Report
Mon 6 Feb 2017
SRR2079243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3762010
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.031.4542871
GTATCAA44950.029.6474951
TCAACGC53300.025.1159614
ATCAACG54750.024.5768263
CAACGCA55450.024.1421225
TATCAAC56300.023.3697912
AACGCAG59500.022.8467566
GTACATG79100.019.4295831
ACGCAGA71450.018.735667
CGCAGAG71450.018.3496028
TACATGG81450.018.2736132
ACATGGG84150.017.1792893
GTGTAGC19350.016.2786141
AGAGTAC69050.015.54505311
GAGTACT34700.015.31684812
GTATAAT12650.015.0497461
GCAGAGT81050.014.4788129
CAGAGTA79050.014.36446710
GAGTACA60400.014.3271571
TAGCCCT23150.014.1585224
Produced by FastQC (version 0.11.5)