FastQCFastQC Report
Mon 6 Feb 2017
SRR2079243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3762010
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72600.040.9144631
GGTATCA29050.038.6863331
TATCAAC102250.028.9950262
ATCAACG104750.028.2700733
TCAACGC105600.028.2059364
CAACGCA109900.027.1023375
AACGCAG113900.025.9728766
ACGCAGA132250.022.0227287
CGCAGAG134150.021.6050478
GAGTACT91400.020.72436512
AGAGTAC129100.020.36497111
AGTACTT97750.019.44530913
CAGAGTA139200.019.15999210
GCAGAGT142750.018.9977199
GTACTTT99500.018.9339814
GTACATG85650.018.5716631
TACATGG84000.018.366312
ACATGGG90000.016.5666583
TACTTTT118500.015.89836515
ACTTTTT124300.015.68395116
Produced by FastQC (version 0.11.5)