FastQCFastQC Report
Mon 6 Feb 2017
SRR2079242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3517532
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40450.033.8768921
GGTATCA20800.031.1103691
TCAACGC49850.026.7135374
ATCAACG49600.026.7090723
TATCAAC51900.025.6850472
CAACGCA51350.025.5972775
AACGCAG54550.023.9059666
ACGCAGA60900.020.6759877
CGCAGAG62600.019.838968
AGAGTAC61700.017.95466811
GTACATG72300.017.182371
GTGTAGC17450.016.8558391
CAGAGTA66950.016.80445910
GCAGAGT70150.016.4314679
TACATGG70850.016.4267062
GTATAAT13350.015.55238151
ACATGGG76800.015.4527753
TAGCCCT20000.014.6621674
GAGTACT30950.013.8261112
GTTGCGC1750.00113621913.7990998
Produced by FastQC (version 0.11.5)