FastQCFastQC Report
Mon 6 Feb 2017
SRR2079242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3517532
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64350.038.9578321
GGTATCA26250.037.1471141
TCAACGC89450.028.087664
ATCAACG90400.027.9833763
TATCAAC89200.027.895552
CAACGCA93200.026.8834655
AACGCAG97450.025.9297776
ACGCAGA113650.021.4071837
CGCAGAG115350.020.939728
GAGTACT78450.019.66041412
AGAGTAC111800.019.53619411
CAGAGTA117950.018.8978610
AGTACTT83700.018.58737213
GCAGAGT124850.018.3785489
GTACATG75800.018.2928141
GTACTTT85950.017.90393414
TACCGTA2156.582377E-817.6560697
TACATGG76200.017.2104872
ACATGGG79650.016.3350373
CCTATAC8450.015.928413
Produced by FastQC (version 0.11.5)