FastQCFastQC Report
Mon 6 Feb 2017
SRR2079241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682453
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.035.6107521
GTATCAA45350.033.391871
TCAACGC52550.026.9823994
ATCAACG53300.026.8616313
CAACGCA54750.026.02425
TATCAAC55900.025.8181042
AACGCAG57450.024.5609256
ACGCAGA64850.021.2791067
CGCAGAG66250.020.3615328
GTACATG69750.020.0253941
AGAGTAC62650.018.89533811
TACATGG70900.018.4079512
CAGAGTA68250.017.85063410
GCAGAGT72900.017.6118559
GAGTACT30550.017.39669612
GTGGTAT10850.017.2071111
ACATGGG75150.016.8939153
GTATAGG6500.016.4889221
AGTACTT35350.015.32736713
GTGTAGC10500.014.8172341
Produced by FastQC (version 0.11.5)