FastQCFastQC Report
Mon 6 Feb 2017
SRR2079241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682453
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.10993668854589436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.043.1719551
GTATCAA71300.041.125221
TCGCGAC400.002598550434.49566736
TATCAAC99250.029.6251012
ATCAACG103650.028.4340883
TCAACGC103500.028.3419254
CAACGCA107200.027.2671285
AACGCAG109900.026.4760726
ACGCAGA126150.022.7039347
CGCAGAG129400.022.0508238
GAGTACT91100.021.92874512
AGAGTAC126900.020.93546711
AGTACTT96700.020.5483413
CAGAGTA134700.020.1842310
GCAGAGT137850.019.9482659
GTACTTT99900.019.79133614
GTACATG71800.019.0709651
TCGCTAG950.00784278518.15561342
TACATGG74300.017.8358252
TACTTTT110800.017.65750315
Produced by FastQC (version 0.11.5)