FastQCFastQC Report
Mon 6 Feb 2017
SRR2079240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041197
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.034.284621
GTATCAA38400.033.7782441
TCAACGC44950.028.6262454
ATCAACG45800.028.1702923
CAACGCA47150.027.217395
TATCAAC49450.026.042222
AACGCAG49550.025.7598486
ACGCAGA55950.022.134647
CGCAGAG58250.021.1425578
GTACATG65800.018.9765971
AGAGTAC56100.018.82755711
TACATGG66850.017.8177622
CAGAGTA61100.017.62579710
GAGTACT25750.017.15805812
GCAGAGT66000.016.944529
ACATGGG70750.016.2368973
GTATAGC9100.015.9641161
AGTACCG3603.6379788E-1115.3321375
ACCGTAT2503.6778874E-715.1788168
GTATAGA10050.015.1434081
Produced by FastQC (version 0.11.5)