FastQCFastQC Report
Mon 6 Feb 2017
SRR2079240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041197
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.039.598041
GTATCAA55550.039.413141
TATCAAC77550.028.1272282
ATCAACG79100.027.9251293
TCAACGC80000.027.3952584
CAACGCA82750.026.2762995
AACGCAG86050.025.1528346
GTATACG1304.8190795E-621.2847251
ACGCAGA102300.020.6113877
CGCAGAG103800.020.2098088
GTACATG71950.019.5652331
GAGTACT65500.019.33153712
TACATGG74250.018.686212
AGAGTAC99400.018.67400711
GTACTTT71400.017.88025714
AGTACTT71250.017.8166513
CAGAGTA105900.017.72329910
TATACCG2751.9645086E-1017.5706185
ACATGGG77550.017.5350113
GCAGAGT111950.017.2277839
Produced by FastQC (version 0.11.5)