FastQCFastQC Report
Mon 6 Feb 2017
SRR2079239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585080
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45800.036.0344851
GGTATCA22650.034.217261
TCAACGC58850.027.6693234
ATCAACG61100.026.7068673
CAACGCA61450.026.4986115
TATCAAC61450.026.2982712
AACGCAG64800.024.7560276
ACGCAGA76400.020.5905727
CGCAGAG79000.019.782738
GTACATG89200.019.5880831
AGAGTAC71250.018.8437711
TACATGG93850.018.4702432
ACATGGG96950.017.7564333
CAGAGTA81450.016.95008910
GCAGAGT87350.016.4769279
TCTATAC9050.016.3916593
ACCGTGC8600.016.0463668
CATGGGG72800.015.5907684
GAGTACT30800.015.3512112
GTATACG1657.120292E-414.6784271
Produced by FastQC (version 0.11.5)