FastQCFastQC Report
Mon 6 Feb 2017
SRR2079239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585080
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65850.042.118841
GGTATCA26650.040.74651
TCAACGC96750.028.755234
TATCAAC96000.028.6562822
ATCAACG98350.028.5681953
CAACGCA99600.027.7591345
AACGCAG103700.026.5669546
ACGCAGA125650.021.451957
CGCAGAG128550.020.9093518
AGAGTAC123900.020.13411
GAGTACT82950.020.04904712
GTACATG90750.019.4729651
CAGAGTA132100.019.04091510
AGTACTT87800.018.89856513
GTACTTT88350.018.82390214
TACATGG90550.018.4497152
GCAGAGT139800.018.4119939
ACATGGG98100.016.7482993
TACTTTT100000.016.49313215
ACTTTTT109000.016.20737316
Produced by FastQC (version 0.11.5)