FastQCFastQC Report
Mon 6 Feb 2017
SRR2079238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480267
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39150.035.7045251
TCAACGC50700.027.9645064
CAACGCA52050.027.305875
GGTATCA20300.027.2707841
ATCAACG52600.027.0855483
TATCAAC52750.026.117012
AACGCAG55850.025.386236
ACGCAGA65700.020.3197757
CGCAGAG68100.019.249358
GTACATG77500.018.9740871
AGAGTAC63150.018.41560211
TACATGG78800.018.0089592
CAGAGTA69300.017.17968810
GTGTAGC15150.017.1286181
ACATGGG80700.016.842093
GAGTACT30400.016.2320412
GCAGAGT76450.016.2044359
GTATAGG9500.014.5683411
CATGGGG55600.014.5182534
TATACAG17250.014.1987075
Produced by FastQC (version 0.11.5)