FastQCFastQC Report
Mon 6 Feb 2017
SRR2079238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480267
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64450.041.3752671
GGTATCA26450.037.0058251
ATCAACG94350.028.5676733
TATCAAC93250.028.4605462
TCAACGC95350.028.159484
CAACGCA98950.027.1349815
AACGCAG102950.025.9506976
ACGCAGA121400.021.490067
CGCAGAG123800.020.8474548
GAGTACT85700.020.53283712
GTACATG79400.020.2554441
AGAGTAC117600.020.06819711
TAGTCGG2603.6379788E-1219.90112940
AGTACTT92300.019.13580513
TACATGG80900.019.068862
GTACTTT92400.018.96984714
CAGAGTA126100.018.85227410
GCAGAGT130100.018.4582949
CCGATCG950.00783334518.159599
ACATGGG86150.017.265843
Produced by FastQC (version 0.11.5)