FastQCFastQC Report
Mon 6 Feb 2017
SRR2079237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944601
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41450.034.3108251
GGTATCA21950.031.213651
TCAACGC50700.027.7624724
ATCAACG51850.027.3463293
TATCAAC52950.026.5415122
CAACGCA53300.026.472935
AACGCAG55350.025.1808096
ACGCAGA63550.021.3888077
CGCAGAG64550.020.8974768
AGAGTAC62750.019.634311
CAGAGTA69000.018.20594410
GTACATG67050.017.3918191
GCAGAGT73100.017.2314439
GAGTACT31800.016.71186812
TACATGG68450.016.0921632
GTATAGC11050.015.6574721
GTGTACG2907.2486728E-915.5116941
AGTACTT34400.015.14781213
GTACTTT35100.015.14066614
ACATGGG71450.015.0646253
Produced by FastQC (version 0.11.5)