FastQCFastQC Report
Mon 6 Feb 2017
SRR2079237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944601
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.041.8969961
GTATCAA63450.040.117261
TATCAAC89000.028.4669972
ATCAACG91050.028.1293413
TCAACGC90800.028.016724
CAACGCA93850.027.253335
AACGCAG99850.025.65466
ACGCAGA114850.021.9951617
GAGTACT78750.021.5566112
CGCAGAG117100.021.2742188
AGAGTAC112500.020.18084311
AGTACTT85850.020.01089313
GTACTTT86300.019.75336514
GCAGAGT121650.019.4003669
CAGAGTA118150.019.39100510
GTACATG68950.017.8566841
TACTTTT97550.017.6881515
ACTTTTT102150.017.6007716
TACATGG70900.016.7474172
TATACCG2252.0397383E-615.340985
Produced by FastQC (version 0.11.5)