FastQCFastQC Report
Mon 6 Feb 2017
SRR2079236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379031
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56150.033.9438061
GGTATCA28250.032.937641
TCAACGC69500.027.154284
ATCAACG70650.026.8099483
CAACGCA71350.026.256795
TATCAAC73700.025.488222
AACGCAG75900.024.9100486
ACGCAGA88150.020.7049477
CGCAGAG89100.020.2135898
GTACATG97200.019.9287661
TACATGG100500.018.279052
AGAGTAC86100.017.83903311
ACATGGG103900.017.4665033
CAGAGTA95250.016.56020410
GAGTACT45050.016.47094212
GTGTAGC22850.016.0464271
TAGCCCT23700.016.0132754
GCAGAGT100950.016.001079
CATGGGG76400.014.9475654
GTGGTAT17700.014.852481
Produced by FastQC (version 0.11.5)