FastQCFastQC Report
Mon 6 Feb 2017
SRR2079236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379031
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85000.041.4503971
GGTATCA34950.040.1654171
TATCAAC126300.027.952232
ATCAACG128650.027.3343373
TCAACGC129600.027.0540894
CAACGCA131050.026.6494145
AACGCAG136500.025.5403696
ACGCAGA159950.021.511717
CGCAGAG164100.020.7961188
AGAGTAC159550.019.63671111
GAGTACT115700.019.38472612
CAGAGTA170400.018.69010710
AGTACTT122650.018.53524613
GCAGAGT173800.018.5230089
GTACTTT122250.018.42868814
GTACATG96100.018.2773721
TACATGG97100.017.379362
TACTTTT136800.016.443415
ACTTTTT145600.016.25521916
ACATGGG102200.016.1744233
Produced by FastQC (version 0.11.5)