FastQCFastQC Report
Mon 6 Feb 2017
SRR2079235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772453
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40750.031.9174881
GGTATCA21650.029.8780461
TCAACGC49250.026.3398884
ATCAACG50350.026.0385273
CAACGCA51600.025.1402995
TATCAAC52400.024.5788362
AACGCAG55350.023.312366
ACGCAGA62700.020.0293167
GTACATG77750.019.2644211
CGCAGAG64150.019.2541528
TACATGG76300.018.5542372
AGAGTAC60100.017.68834111
ACATGGG82450.016.779773
CAGAGTA66650.016.51967210
GAGTACT29400.015.84799312
GCAGAGT71900.015.6498839
GTATAGA11300.015.3059431
GTGTAGC19150.014.9926291
TATACCG2808.210009E-814.7861645
CATGGGG58150.014.239474
Produced by FastQC (version 0.11.5)