FastQCFastQC Report
Mon 6 Feb 2017
SRR2079235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772453
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66500.039.8999371
GGTATCA26550.037.7860951
ATCAACG92900.028.7167973
TCAACGC94400.028.2239324
TATCAAC94400.028.0045762
CAACGCA97100.027.33255
AACGCAG102100.025.9305066
ACGCAGA121900.021.430387
CGCAGAG123450.020.9620138
GAGTACT84350.019.51174512
AGAGTAC118900.019.18149611
TATACCG1801.9769686E-719.1734265
CAGAGTA127350.018.26096710
GTACATG79350.018.1797391
GCAGAGT131400.018.1707789
AGTACTT90100.017.88051613
GTACTTT91450.017.65849714
TACCGTA2751.9645086E-1017.5684347
TACATGG80850.017.3307862
ACATGGG85900.016.0708583
Produced by FastQC (version 0.11.5)