FastQCFastQC Report
Mon 6 Feb 2017
SRR2079234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673439
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43500.031.1143021
GGTATCA24000.030.8656251
TCAACGC53050.024.4521714
ATCAACG53650.024.1787093
CAACGCA54900.023.6913555
TATCAAC55950.023.5785032
AACGCAG58150.022.3076136
GTACATG78500.019.8874651
CGCGATA900.005730760319.1701449
ACGCAGA65050.019.1453367
CGCAGAG67450.018.0037868
TACATGG80250.017.9880522
ACATGGG83350.017.094623
GTGTAGC17450.016.8614941
AGAGTAC63100.016.51802811
CAGAGTA69400.015.41639610
GTATATA17350.015.16305351
GCAGAGT76200.014.9465059
TATACTG16400.014.5153055
CATGGGG63350.014.4860524
Produced by FastQC (version 0.11.5)