FastQCFastQC Report
Mon 6 Feb 2017
SRR2079234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673439
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68450.040.5772971
GGTATCA26600.037.450011
ATCAACG94800.029.0504423
TATCAAC95350.028.8104842
TCAACGC95250.028.7320354
CAACGCA99250.027.3654375
AACGCAG103800.026.2034236
ACGCAGA121200.021.980497
CGCAGAG124950.021.1243278
AGAGTAC120200.020.15015811
GAGTACT86500.019.9833112
CAGAGTA127750.019.25637210
AGTACTT92050.018.81285913
GCAGAGT134300.018.702829
GTACATG79900.018.6582761
TACATGG81400.017.9762732
GTACTTT97550.017.86384614
ACATGGG85900.017.0747343
TACTTTT108150.015.89027615
ACTTTTT119450.015.45575916
Produced by FastQC (version 0.11.5)