FastQCFastQC Report
Mon 6 Feb 2017
SRR2079233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081001
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35550.030.8407141
GGTATCA18450.030.5559671
TCAACGC44050.024.5148874
ATCAACG44200.024.1975213
CAACGCA45500.023.8098565
TATCAAC47550.023.0182232
AACGCAG48500.022.4082186
ACGCAGA54600.019.2719027
CGCAGAG55200.018.6249248
GTGTAGC16250.018.5170021
GTACATG63500.017.1570111
GTATAGC11300.016.8340661
AGAGTAC52700.016.634511
TAGCCCT19300.016.4460664
TACATGG63850.016.1686342
GTATTAG7300.016.1087111
GTGTAGA15750.015.5913091
GCAGAGT61200.015.5655859
CAGAGTA59100.015.53391110
AGGACCG4550.015.1655135
Produced by FastQC (version 0.11.5)