FastQCFastQC Report
Mon 6 Feb 2017
SRR2079233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081001
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.039.3466341
GTATCAA58900.038.5251851
ATCAACG81350.027.3196433
TCAACGC81800.027.1271614
TATCAAC82400.027.0133972
CAACGCA83450.026.5907945
AACGCAG86950.025.325286
ACGCAGA102700.021.2345967
CGCAGAG104250.020.65148
AGAGTAC101850.019.47815311
CAGAGTA108050.018.9033110
GAGTACT75300.018.87744512
AGTACTT79250.018.0212813
GCAGAGT114600.017.943319
GTATAGG8900.017.4895521
GTACTTT81850.017.36847914
CGTATAC1804.1890653E-617.2550683
CGAATCG1402.0857931E-417.24806837
GTACATG64800.017.1350821
GCGTATA1855.457874E-616.78217967
Produced by FastQC (version 0.11.5)