FastQCFastQC Report
Mon 6 Feb 2017
SRR2079232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143436
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.031.530361
GTATCAA32250.029.7240241
ATCAACG37350.024.8467313
TCAACGC38250.024.2621024
TATCAAC39200.023.6075252
CAACGCA40050.022.7416935
AACGCAG42650.021.2744396
ACGCAGA49850.018.062717
CGCAGAG50750.017.8789128
GTACATG62500.017.4970421
TACATGG63200.016.0631962
GTCCTAG9600.015.8613081
GTGTAGC15650.015.4789421
AGAGTAC48300.015.43479411
TAGGACG1605.7631667E-415.093354
ACATGGG66800.015.0287943
TGGACCG3808.367351E-1114.5263925
GTACTAG6750.014.3552841
CAGAGTA54400.014.27505810
TAGCCCT19650.014.2209974
Produced by FastQC (version 0.11.5)