FastQCFastQC Report
Mon 6 Feb 2017
SRR2079232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143436
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52250.039.3941841
GGTATCA21650.037.0707661
ATCAACG73400.027.9785943
TCAACGC73100.027.951774
TATCAAC73250.027.941652
CAACGCA74500.027.2411865
AACGCAG78900.025.6848286
CGCAGAG92150.021.3435678
ACGCAGA92350.021.340487
GAGTACT64600.020.19048512
AGAGTAC92400.019.41864211
CAGAGTA99600.018.53454610
GCAGAGT101550.018.2126169
AGTACTT72100.018.13346913
GTACTTT71450.018.10790314
CGTGCGC4250.017.86163710
GTACATG63050.016.78951
TACATGG62200.016.0920942
GCGTGCG4750.015.25503359
ACTTTTT90050.015.172029516
Produced by FastQC (version 0.11.5)