FastQCFastQC Report
Mon 6 Feb 2017
SRR2079231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725235
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48900.034.9545061
GGTATCA23200.030.2756251
TCAACGC58800.028.8664654
ATCAACG59550.028.7925723
CAACGCA59850.028.245135
AACGCAG63350.026.4123346
TATCAAC64200.026.407762
ACGCAGA74650.021.9052647
CGCAGAG76900.020.8617138
GTACATG96650.020.7640081
TACATGG99000.019.4269712
AGAGTAC72350.019.17774411
ACATGGG103200.018.1854923
CAGAGTA80600.017.55734410
GCAGAGT85100.016.9527139
CGAATCG2057.779945E-716.8262737
GAGTACT31400.016.15685712
CATGGGG77300.015.6204814
CTATACT10850.014.6262824
GTACTTT37250.014.5457714
Produced by FastQC (version 0.11.5)