FastQCFastQC Report
Mon 6 Feb 2017
SRR2079231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725235
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.040.710031
GTATCAA75250.040.342071
TATCAAC110450.027.3442042
ATCAACG111650.026.895743
TCAACGC112350.026.6359964
CAACGCA114400.026.0078335
AACGCAG120400.024.7174056
CGTGCCG3900.021.23389410
ACGCAGA139550.021.119947
CGCAGAG143600.020.4248628
GAGTACT94350.019.45596112
AGAGTAC141500.019.23993311
TATACCG2002.8701834E-818.983915
GTACATG97750.018.6054081
CAGAGTA150050.018.48855810
GCAGAGT154750.018.19469
AGTACTT101700.018.18092713
GTACTTT103350.017.86400214
TACATGG98700.017.7302062
ACATGGG105650.016.4005033
Produced by FastQC (version 0.11.5)