FastQCFastQC Report
Mon 6 Feb 2017
SRR2079230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3486063
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46650.033.070741
GGTATCA22750.031.3217891
TCAACGC55150.026.834474
ATCAACG56050.026.7113193
CAACGCA58500.025.1802185
TATCAAC59800.024.9990392
AACGCAG61700.024.2097436
ACGCAGA69800.020.5112727
CGCAGAG71100.019.8936398
AGAGTAC67750.018.0834411
CAGAGTA74450.016.73418210
GTGTAGC18200.016.5351661
GTACATG76600.016.4825651
TACATGG73450.016.3107622
GCAGAGT79250.016.2427339
GAGTACT35550.016.0167812
GTACTAG7750.015.17532351
GTATAGA11000.015.0941711
ACATGGG78450.014.9508963
ACCGTAC1951.6003713E-414.1532238
Produced by FastQC (version 0.11.5)