FastQCFastQC Report
Mon 6 Feb 2017
SRR2079230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3486063
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.038.945331
GTATCAA68150.038.3726271
ATCAACG95850.027.5849693
TATCAAC94650.027.5335482
TCAACGC95600.027.440474
CAACGCA99650.025.9788445
AACGCAG102600.025.1685776
ACGCAGA122000.020.7922297
CGCAGAG124400.020.2751738
GAGTACT91350.019.29989812
AGAGTAC121900.019.24632611
CAGAGTA130250.018.19791610
GCAGAGT133250.017.9694569
GTACTTT100200.017.80416514
AGTACTT98900.017.75397713
GTATAGC10550.017.7054461
GTACATG75650.017.6956941
TACATGG74200.017.2586122
TACCGTA1653.8335013E-516.7332237
ACATGGG79600.015.7408943
Produced by FastQC (version 0.11.5)