FastQCFastQC Report
Mon 6 Feb 2017
SRR2079229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887395
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.034.5989271
GTATCAA39600.032.3272821
ATCAACG49600.025.4611953
TCAACGC49100.025.2988284
TATCAAC49450.025.2825452
CAACGCA49750.024.7602215
AACGCAG51850.023.6908516
ACGCAGA59500.020.1788567
GTACCGT2850.019.371116
CGCAGAG62350.018.8691438
GTACATG64750.017.9540391
AGAGTAC57500.017.5854911
TACATGG63850.017.5792732
ACATGGG68400.016.6974953
GAGTACT30050.016.19223412
CAGAGTA65050.016.12801710
GCAGAGT68650.015.5838889
GTATATG10650.015.269011
TGTACCG4400.014.89979655
AGTACTT34000.014.71680913
Produced by FastQC (version 0.11.5)