FastQCFastQC Report
Mon 6 Feb 2017
SRR2079229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887395
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60400.042.427041
GGTATCA22500.037.3495371
ATCAACG87900.029.4090293
TCAACGC88050.029.0845474
TATCAAC87650.028.9022672
CAACGCA89000.028.2699685
AACGCAG92400.027.2358656
ACGCAGA109100.022.5519897
CGCAGAG110400.022.033328
GAGTACT76550.021.22929612
AGAGTAC104700.021.05785211
AGTACTT80600.020.41481413
CAGAGTA111200.020.04414710
GCAGAGT115100.019.6047949
GTACTTT86400.019.36746814
GTACATG63800.018.7549931
TACATGG64350.017.7528132
TACTTTT96500.017.05496815
ACATGGG69500.016.8842533
ACTTTTT104100.016.83731716
Produced by FastQC (version 0.11.5)