FastQCFastQC Report
Mon 6 Feb 2017
SRR2079228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435180
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.036.165521
GTATCAA53350.033.526091
ATCAACG66500.026.0942213
TCAACGC66850.025.9059984
CAACGCA68250.025.2734995
TATCAAC69250.025.179262
AACGCAG71600.023.9946446
GTACATG100250.020.671371
ACGCAGA82100.020.5471257
CGCAGAG82250.019.9644078
TACATGG101250.019.608492
AGAGTAC76900.018.57987611
GTGTAGC15300.018.3155691
ACATGGG106500.018.301933
CAGAGTA83950.017.80065710
GCAGAGT87500.017.473399
CATGGGA45200.015.8753364
GAGTACA75200.014.5837161
CATGGGG67100.014.1386584
TAGTCGG2454.9660575E-614.08074440
Produced by FastQC (version 0.11.5)