FastQCFastQC Report
Mon 6 Feb 2017
SRR2079228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435180
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34580.10066430288951381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.040.1340521
GTATCAA82450.039.756241
TATCAAC116150.028.168682
ATCAACG119150.027.5463373
TCAACGC122300.026.7239654
CAACGCA124450.026.262285
AACGCAG128550.025.2930076
ACGCAGA154150.020.7491237
CGCAGAG155700.020.2745768
GAGTACT100950.019.85587712
AGAGTAC148500.019.53839111
CAGAGTA156350.018.82220310
AGTACTT105800.018.81330913
CGTTCGT1301.18294665E-418.57476840
GCAGAGT163250.018.4281869
GTACATG101200.018.245191
GTACTTT110150.018.22984914
TACATGG102100.017.6112162
TACTTTT122200.016.51740615
ACTTTTT130100.016.3365616
Produced by FastQC (version 0.11.5)